for females ages 18-55 (full criteria)
Healthy Volunteers
healthy people welcome
study started
estimated completion
Principal Investigator
by Nasim C Sobhani, MD (ucsf)



This study evaluates the use of metagenomic next generation sequencing in identifying microbial DNA in plasma samples of patients with preterm premature rupture of membranes.

Official Title

Using Metagenomic Next-generation Sequencing to Identify Microbial DNA in Maternal Plasma in Cases of Preterm Premature Rupture of Membranes


Although preterm premature rupture of membranes (PPROM) occurs in only 3% of pregnancies, it accounts for 30% of preterm births (PTB) and is associated with serious maternal and neonatal morbidity. An important factor in the underlying pathophysiology of PPROM and subsequent PTB is subclinical infection, which promotes a cascade of events that contribute to synthesis of prostaglandins, release of proinflammatory cytokines, infiltration of neutrophils, and activation of metalloproteases. Over time, enhanced activity of these infectious and inflammatory pathways contributes to the development of spontaneous labor and/or overt intraamniotic infection (IAI). Unfortunately, the majority of patients with PPROM do not manifest signs and symptoms of infection that are detectable by clinical examination, laboratory evaluation, and traditional microdiagnostic tests, and attempting to predict length of latency period and/or timing of delivery remains a clinical challenge. We propose the use of metagenomic next-generation sequencing (mNGS) to identify microbial DNA in maternal plasma following PPROM. We hypothesize that the presence and abundance of microbial DNA is associated with a shorter latency period and that an increase in the abundance of microbial DNA precedes delivery.


Preterm Rupture of Membranes, Premature Birth, Premature Rupture Fetal Membranes, Rupture, mNGS, PPROM


You can join if…

Open to females ages 18-55

  • For PPROM group, preterm premature rupture of membranes between 16 0/7 and 33 6/7 weeks of gestation
  • For control group, healthy pregnancy with no evidence of preterm premature rupture of membranes or other major complications

You CAN'T join if...

  • Maternal age < 18 years
  • Major fetal congenital malformation


  • University of California, San Francisco accepting new patients
    San Francisco California 94158 United States

Lead Scientist at University of California Health

  • Nasim C Sobhani, MD (ucsf)
    Assistant Professor, Ob/Gyn, Reproductive Sciences, School of Medicine. Authored (or co-authored) 20 research publications


accepting new patients
Start Date
Completion Date
University of California, San Francisco
Study Type
Expecting 25 study participants
Last Updated