Summary

for people ages 18 years and up (full criteria)
healthy people welcome
at UC Irvine
study started
estimated completion:
Kristen Kelly (uci)

Description

Summary

The researchers believe that pro-angiogenic factors are upregulated in a wide range of dermatologic diseases including port wine stains, hemangiomas, angiofibromas, Kaposi's sarcoma, angiosarcoma, scars, rosacea and psoriasis. The researcher can perform immunohistochemistry and/or microarray analysis and/or quantitative polymerase chain reaction on previously biopsied skin specimens and newly biopsied skin specimens to evaluate the expression of various angiogenic factors in these dermatologic diseases. In addition, some of the skin specimens may be utilized to make cell cultures to study expression of angiogenic factors and interactions of cells in dermatologic disease.

Details

Previously or newly collected biospecimens from various dermatologic diseases including port wine stains, hemangiomas, angiofibromas, Kaposi's sarcoma, angiosarcoma, scars, rosacea and psoriasis will be evaluated for markers of angiogenesis. Additionally, researchers can use discarded human skin tissue samples from skin biopsy/surgery sites which are removed for closure but are not submitted for histopathologic analysis.

Currently, biospecimens prospectively collected are only from lesions with a known diagnosis: 1) port wine stains, hemangiomas, cherry angiomas, facial angiofibromas, scars and psoriasis these lesions are generally not biopsied for diagnosis or 2) previously biopsied and diagnosed other vascular lesions such as angiosarcomas and Kaposi's Sarcoma.

Specimens collected will be processed for microarray analysis, qPCR and/or immunohistochemistry performed to evaluate expression of various angiogenic factors and their receptors including: vascular endothelial growth factor, basic fibroblast growth factor, angiopoietin 1, angiopoietin 2, matrix metalloproteinase, tissue inhibitor metalloproteinase I and thrombospondin-1, Angiotensin 2 receptor.

Additional, tissue samples will be used to isolate three cell types: endothelial cells, keratinocytes, and fibroblasts. Tissue samples will be digested to isolate the cells which will be cultured separately and then incorporated into an in-vitro model to observe how blood vessels form in skin affected by port wine stains as compared to vessel growth in unaffected skin.

Keywords

Dermatologic Diseases Skin Diseases skin tissue sample

Eligibility

You can join if…

Open to people ages 18 years and up

  • 18 years of age or older.
  • willing to have a skin biopsy

You CAN'T join if...

  • under 18 years of age
  • unable to carry out instructions

Locations

  • Beckman Laser Institute Medical clinic accepting new patients by invitation only
    Irvine California 92612 United States
  • Gottschalk Medical Plaza accepting new patients
    Irvine California 92612 United States

Lead Scientist

  • Kristen Kelly (uci)
    Professor, Dermatology. Authored (or co-authored) 85 research publications

Details

Status
accepting new patients
Start Date
Completion Date
(estimated)
Sponsor
University of California, Irvine
ID
NCT00842283
Study Type
Observational
Last Updated